BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC367740 |
| Description | Mycoplasma Broth without Glucose |
| Composition | Porcine gastric digest:10g, Beef extract:5g, Yeast extract:5g, NaCl:2.5g, Phenol red:0.02g, Horse serum:200ml, Penicillin:800, 000 units, pH:7.6±0.2(25℃) |
| Growth conditions | 37°C; 2-3 days; Microaerophilic (3%-5% O₂, 10% CO₂); |
| Subculture procedure | Remove one vial of frozen ureaplasma stock (500 μL) and six tubes containing prepared medium (one as control; 5 for culture). Add all thawed ureaplasma to the first tube (containing 4.5mL prepared ureaplasma medium), mix thoroughly, then transfer 0.5mL to the next culture tube. Repeat to create 5 dilution gradients. Incubate all 5 diluted tubes and 1 control tube at 37°C under aerobic conditions for 18 hours. |
| Storage conditions | liquid nitrogen |
| Sharing mode | Public welfare sharing |
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