Chlamydia trachomatis serovar A|362969 |BNCC

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Chlamydia trachomatis Serovar A

Culture medium	BNCC366598
Description	NK-92MI Cell-Specific Medium
Composition	84%MEM-α+0.2 mM Inositol+0.1 mM β-Mercaptoethanol+0.02 mM Folic Acid+12.5% HS+12.5% FBS+1% Penicillin-Streptomycin
Growth conditions	37°C; microaerophilic (3%-5% O₂, 10% CO₂);
Subculture procedure	①Inoculation procedure: 24-48 hours before inoculation, transfer the frozen host cells into T25 flask, remove the cell culture medium when the host cell density reaches 80-90%, and inoculate 100-500 μL of virus solution. Adsorb under growth conditions for 1-2 hours, gently shake the T25 flask every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cytopathy; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution.
Storage conditions	liquid nitrogen
Sharing mode	Public welfare sharing