Burkitt's lymphoma cells|100223 |BNCC

Essential Information References Certificate Related Products

Daudi

Subculture procedure	Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 6-7mL of complete medium and place the flask vertically in an incubator. Cell passage: ① Centrifuge method: Collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly. Divide the cell suspension into fresh T25 flasks containing 8mL of culture medium at a ratio of 1:2.② Half volume liquid exchange method: Please gently aspirate half of the supernatant culture medium, resuspend the remaining culture medium with cell sediment, and divide the cell suspension into fresh T25 flask containing 8mL of culture medium in a ratio of 1:2.③ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches greater than 2 × 10^6 cells/mL.
Growth conditions	37°C; 5% CO₂ + 95% air
Safety level	0
morphology	Lymphoblast-like，Round
Sharing mode	Public welfare sharing

Daudi human Burkitt ' S lymphoma cell

Suspension, lymphoblast-like

No. 100223

Product format: 2ml frozen vial x 2, or centrifuge tube 15mL

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receipt Instructions: If any abnormality is found on the day of receipt, please contact customer service within 24 hours. Overdue goods are considered to be well received; in the form of cryopreservation tubes, they should be stored in a -80°C refrigerator in time after receipt. If they are not used for a long time, they should be Transfer to liquid nitrogen for storage overnight; centrifuge tubes are 15mL. After receiving the products, the centrifuge tubes are placed in the original incubator for 4 hours, and then the cells are routinely operated. During resuscitation, each tube should not be kept after one use. Please operate in strict accordance with this instruction, otherwise, the reissue service will not be provided due to the inactivation of cells.

Growth conditions:37 ℃,5% CO₂,CM2-1 culture solution. CM2-1 culture solution: 90% RPMI-1640 + 10% FBS. RPMI-1640:1640 culture solution, containing glutamine.

Recovery steps:

① 1 new 100mm plate containing 12mL of the above culture solution;

② Take out the frozen storage tube from liquid nitrogen or -80 ℃, take a water bath at 37 ℃ for 1~2min, and move it into the safety cabinet for recovery as soon as possible after it is completely dissolved.

③ Use a sterile straw to suck the dissolved liquid into the new plate and shake it clockwise;

④ Put it into an incubator (37 ℃,5% CO₂), change the liquid overnight, and it will be full in 6-7 days.

Subculture: gently blow the cultured suspension cells evenly, distribute them to 3-6 fresh culture liquid dishes, gently shake well and put them into incubator for culture;

Cryopreservation : transfer the suspended cells to a centrifuge tube, centrifuge (110g,3min), resuspend the cells with 3mL of frozen storage solution (90% FBS + 10% DMSO) after centrifugation, blow evenly, divide them into 3 frozen storage tubes, and freeze storage at -80 ℃ with a program cooling box.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

Item	quality standard	recovery record
viability:	suspension growth rate ≥ 80.0% in 168 hours	inoculation with 20% cell solution, suspension growth rate reaches 30.0% in 18 hours, and 80.0% in 160 hours
cell morphology:	suspension, lymphoblast-like	CM2-1 culture medium, suspended, lymphoblast-like, round, few clusters grow
attached figure:
Conclusion:	good viability, and no abnormal cell morphology, qualified

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