Mouse monocyte macrophage leukemia cells|100199 |BNCC

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RAW264.7

Culture medium	BNCC338068
Description	DMEM-H Complete Medium (with 10% FBS)
Composition	90%DMEM-H+10%FBS
Growth conditions	37°C; 5% CO₂ + 95% air
Subculture procedure	Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 5-6mL of complete medium and place the flask in an incubator. Cell passage: ① Use a 5ml pipette to directly aspirate the culture medium and blow the cells; ② Observe under the microscope whether the cells are evenly dispersed. ③ Divide the cell suspension into fresh T25 flasks at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator.; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
morphology	Macrophage-like，Round，Spindle-shaped (short)，irregular edge，Monolayer adherent growth
Sharing mode	Public welfare sharing