Normal prostate epithelial cells|341583 |BNCC

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RWPE-1

Culture medium	BNCC360861
Description	KM Complete Medium
Composition	500ml KM+5mL KGS+5ml P/S
Growth conditions	37°C; 5% CO₂ + 95% air
Subculture procedure	Resuscitation steps: ① Remove the Frozen vial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves, quickly immerse it in a 37 ℃ water bath, shake the Frozen vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Add the cell suspension into a T25 bottle containing 5-6mL of complete culture medium and incubate in a culture incubator. Cell passage: ① Remove the old culture medium, wash twice with PBS, and add 1-2mL trypsin; ② Under the microscope, observe the digestion process. When the cell edge shrinks and the adhesion becomes loose (you can use a straw to suck up some trypsin and gently blow it onto a certain part of the cell layer. The cell layer can be seen to have fallen off with the naked eye, indicating that digestion is complete. Otherwise, continue digestion). Directly suck out the trypsin, add 5-6 milliliters of culture medium containing 10% FBS to terminate digestion, gently blow the cell layer, blow it off, and disperse it. ③ Centrifuge the cell suspension at 1000rpm for 3 minutes, discard the supernatant, resuspend the cells in 1-2mL of complete culture medium, and then add the cell suspension in a 1:2 ratio to a T25 bottle containing 5-6mL of complete culture medium. Mix the cell suspension evenly and culture it in an incubator. ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
morphology	Epithelial-like，Spindle-shaped (short)，irregular edge
Sharing mode	Public welfare sharing