Mouse mononuclear macrophages|338375 |BNCC

Essential Information Certificate Related Products

LADMAC

Subculture procedure	Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 ℃ refrigerator and place it in PE gloves. Quickly immerse it in a 37 ℃ water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 5-6mL of complete medium and place the flask in an incubator. Cell passage: ① Use a 5ml pipette to directly aspirate the culture medium and blow the cells; ② Observe under the microscope whether the cells are evenly dispersed. ③ Divide the cell suspension into fresh T25 flasks at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator.; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%.
Growth conditions	37°C; 5% CO₂ + 95% air
Safety level	0
morphology	Macrophage-like，Round，Single cells，Clean background
Sharing mode	Public welfare sharing

LADMAC mouse mononuclear macrophage

Suspension, macrophage-like

No.: 338375

Product format: 2ml frozen vial x 2, or centrifuge tube 15mL ;

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Growth Condition:37 ℃,5% CO₂ CM2-1 culture solution

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells, it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight. Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 4h, and then carry out handling procedure. During recovery, each vial shall be used up once and shall not be retained. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of lose of cell viability.

Growth conditions:37 ℃,5% CO₂,CM2-1 culture solution. CM2-1 culture solution: 90% RPMI-1640 + 10% FBS. RPMI-1640:1640 culture solution, containing glutamine.

Recovery steps:

(1)Prepare a new 100mm culture dish containing 12ml of the above culture medium;

remove the frozen vial from liquid nitrogen or refrigerator at -80℃, thaw the vial in 37°C water bath for 1-2 minutes. Transfer the vial to biosafety cabinet for culture as soon as the contents are completely thawed.

(2)Draw the solution with a sterile pipette, drip into a new culture dish, and mix it evenly by shaking clockwise;

(3)Put it into incubator (37℃，5%CO₂）, change the media overnight, and it will grow up in 4-6 days.

Subculture: the suspended cells were gently beaten and evenly distributed into 3-6 fresh culture medium dishes. After being shaken well, the cells were put into the incubator for culture

Cryopreservation: The suspended cells were transferred to the centrifuge tube for (110g, 3min) centrifugation，after centrifugationterminate digestion with 3ml of cryopreservation solution （90%FBS+10%DMSO), aspirate and dispense into 3 frozen vials, and cryopreserve at -80 ℃ with a programmed cooling box.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

item	quality standard	recovery record
viability	suspension growth rate ≥ 80.0% in 96 hours	inoculation with 20% cell solution, suspension growth rate reaches 30.0% in 18hours, and 80.0% in 90hours
cell morphology	suspension, macrophage-like	in CM2-1 culture solution, suspended, macrophage-like, irregular round, loose adherent
attached figure
conclusion	good viability, and no abnormal cell morphology, qualified

Download certificate