Mouse bone marrow dendritic cells|359847 |BNCC

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JAWSII

Culture medium	BNCC360904
Description	JAWS II Cell-Specific Medium
Composition	79% MEM-α+20% FBS+5 ng/ml Mouse GM-CSF+1% L-Glutamine
Growth conditions	37°C; 5% CO₂ + 95% air
Attention	If any abnormality is found on the day of receipt, please contact customer service within 24 hours. Failure to do so will be considered as confirmation of good condition upon receipt. For cryovial format: Upon receipt, immediately store at -80°C. For long-term storage, transfer to liquid nitrogen overnight. During resuscitation, use the entire contents of one vial at a time; do not reserve any portion. For T25 flask format: Upon receipt, let the flask stand in the incubator for 2–3 hours before proceeding with routine cell handling. Under sterile conditions, transfer the original culture medium from the flask to a centrifuge tube. Centrifuge at 1000–1200 rpm for 5–10 minutes, then resuspend the cells. Transfer the suspended cells back to the original T25 flask, add complete medium to a total volume of 10 mL, and place the flask in the incubator for cultivation. Please strictly follow the instructions above. Failure to do so may result in cell inactivation, and no replacement will be provided.
morphology	Epithelial-like，Spindle-shaped (short)，Round，irregular edge，Monolayer adherent growth，partially floating growth
Sharing mode	Public welfare sharing