Clostridium perfringens|BNCC185933 |BNCC

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Clostridium perfringens

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h; Anaerobic;
Growth characteristics	G + bacillus, arginine dihydrolase positive, lecithin enzyme positive, lipase negative, urease negative, fermented glucose, trehalose, raffinose, do not ferment arabinose, xylose. Obligatory anaerobic, optimal pH5.5 ~ 8.0.
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Protuberance: Middle convex Surface: Smooth Gray Texture: Wet and Easy to Pick
application	research, quality control. Research, quality control, "GB 4789.13-2012 Clostridium perfringens test" positive control strain, "GB 4789.28 medium and reagent quality requirements" standard strain.
Sharing mode	Public welfare sharing

Clostridium perfringens

Storage conditions: 2~8 ℃

No.: 185933

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, 24-48h, liquid thioglycolate medium: caseptone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g, L-cystine 0.5g, sodium chloride 2.5g, blade azure 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a φ18mm test tube of 10ml liquid medium; and two agar plates(put in an anaerobic environment for deoxygenation in advance)

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Put the test tube under the above culture conditions for cultivation. The culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 24-48h, the liquid medium is turbid, and the strain grow slowly on the plate.
colony morphology: (above)	Clostridium perfringens is a gram-positive bacterium. FT liquid culture is turbid and plate strains are weak. Liquid culture is recommended.
conclusion	good viability, no abnormal colony morphology, qualified