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Saccharomyces NMY51
Storage conditions : 2~8 ℃
No. : 357965
Product format :freeze dried, 200ul
Validity : 6 years
Biosafety level : 1, handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions: 30°C, aerobic, YM medium, 24-48h.
YM medium: yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (liquid medium not included), distilled water 1.0L, pH 6.2±0.2. Sterilize at 121°C for 15min.
Recovery steps:
① Prepare 1 test tube containing 5~10mL liquid medium and 2 plates;
② Open it in the safety cabinet, burn the top with an alcohol lamp, then quickly drip sterile water to break it, and then smash it with tweezers;
③ Pipette 0.5mL of liquid medium into the freeze-drying tube, dissolve it fully and pour it back into the liquid test tube, and mix well;
④ Pipette 0.2mL of bacterial suspension into the plate, spread evenly, repeat twice to obtain two plates;
⑤ Put all the liquid test tubes and plates under the above-mentioned culture conditions, and the bacteria can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
colony morphology
(above)
Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque
color: orange uplift: middle convex surface: smooth texture: moist and easy to stir