BeNa Culture Collection
Culture medium | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | Experimental preparation: Before the experiment, refer to GB4789.10 or GB7918.5 to carry out the enrichment culture of the sample; The various components in the kit are dissolved on the ice before use, slightly mixed and temporarily centrifuged, and then placed on the ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 51 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channels: FAM, VIC. |
Growth conditions | Culture temperature 37 ℃; Culture time 20 hours; Aerobic gas environment; |
Storage conditions | -20 ℃, cold storage |
Safety level | 2 |
Separation substrate | Human lesion |
application | Rapid detection of Staphylococcus aureus in food and cosmetics |
Sharing mode | Public welfare sharing |
Staphylococcus aureus nucleic acid detection kit certificate
1. Product information
Sample name: Staphylococcus aureus nucleic acid detection kit (multiplex fluorescence PCR method)
Sample number: 361200
Sample batch: 220402
2. Product features
description | 1.5mL cryopreservation tube package, containing reaction solution, positive quality control material, negative quality control material, DEP water |
traceability | BNCC238481(ATCC6538) staphylococcus aureus |
product specifications | 1 tube of reaction solution, 1 tube of positive quality control product, 1 tube of negative quality control product, 1 tube of ddH2O water |
field of application | It is suitable for the detection of Staphylococcus aureus in cosmetics, food and other samples |
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use |
3.Instructions
Experiment preparation: Before the experiment, refer to GB4789.10 or GB7918.5 for enrichment culture of samples; dissolve various components in the kit on ice before use, mix gently and centrifuge briefly and place on ice for later use; reagent preparation : Experimental reaction system (25 μL), 18.5 μL of qPCR mixed reaction solution, 1 μL of template, 5.5 μL of sterile ddH2O. Reaction conditions: 95℃ for 1min 1cycle; 95℃ for 10s, 51℃ for 30s for 40cycles; Fluorescence channel: FAM, VIC.
4. Notes
1. The kit should be stored at low temperature in the dark, and the various components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid the generation of air bubbles;
2. Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;
3. Judgment of results: if the Ct value of the test sample is ≥38 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 35, and there are two typical amplification curves, it is judged as positive; If the Ct value of the test sample is >35 and <38, it is recommended that the sample be redone.
5. Preservation and transportation
Long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.
Henan Engineering Technology Research Center of Industrial Microbial Strain
website: www.bncc.org.cn tel: 400-6699-833