Staphylococcus aureus nucleic acid detection kit (multiplex fluorescent PCR method)|BNCC361200 |BNCC

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Staphylococcus aureus nucleic acid detection kit (multiplex fluorescent PCR method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Experimental preparation: Before the experiment, refer to GB4789.10 or GB7918.5 to carry out the enrichment culture of the sample; The various components in the kit are dissolved on the ice before use, slightly mixed and temporarily centrifuged, and then placed on the ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 51 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channels: FAM, VIC.
Growth conditions	Culture temperature 37 ℃; Culture time 20 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	2
Separation substrate	Human lesion
application	Rapid detection of Staphylococcus aureus in food and cosmetics
Sharing mode	Public welfare sharing

Staphylococcus aureus nucleic acid detection kit certificate

1. Product information

Sample name: Staphylococcus aureus nucleic acid detection kit (multiplex fluorescence PCR method)

Sample number: 361200

Sample batch: 220402

2. Product features

description	1.5mL cryopreservation tube package, containing reaction solution, positive quality control material, negative quality control material, DEP water
traceability	BNCC238481(ATCC6538) staphylococcus aureus
product specifications	1 tube of reaction solution, 1 tube of positive quality control product, 1 tube of negative quality control product, 1 tube of ddH2O water
field of application	It is suitable for the detection of Staphylococcus aureus in cosmetics, food and other samples
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3.Instructions

Experiment preparation: Before the experiment, refer to GB4789.10 or GB7918.5 for enrichment culture of samples; dissolve various components in the kit on ice before use, mix gently and centrifuge briefly and place on ice for later use; reagent preparation : Experimental reaction system (25 μL), 18.5 μL of qPCR mixed reaction solution, 1 μL of template, 5.5 μL of sterile ddH2O. Reaction conditions: 95℃ for 1min 1cycle; 95℃ for 10s, 51℃ for 30s for 40cycles; Fluorescence channel: FAM, VIC.

4. Notes

1. The kit should be stored at low temperature in the dark, and the various components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid the generation of air bubbles;

2. Sample processing, reagent preparation, and sample addition should be performed in different areas to avoid cross-contamination;

3. Judgment of results: if the Ct value of the test sample is ≥38 and there is no typical amplification curve, it is judged as negative; if the Ct value of the test sample is less than or equal to 35, and there are two typical amplification curves, it is judged as positive; If the Ct value of the test sample is >35 and <38, it is recommended that the sample be redone.

5. Preservation and transportation

Long-term storage:-20 ℃, valid for 1 year; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833