Aspergillus flavus nucleic acid reference (heat inactivated) (strong positive)|BNCC361768 |BNCC

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Aspergillus flavus nucleic acid reference (heat inactivated) (strong positive)

Culture medium	Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.
Growth conditions	Culture temperature 28 ℃; Culture time 5-10 days; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
application	Pathogenic nucleic acid detection kit cross-reaction, specificity test, and other nucleic acid related studies.
Sharing mode	Public welfare sharing

Aspergillus flavus nucleic acid reference certificate

This reference product was developed by Henan Industrial Microorganisms Engineering Technology Research Center and can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.

1. Product information

product name	Aspergillus flavus nucleic acid reference (heat inactivated) (strong positive)
number	BNCC 361768	batch	220608

2. Product features

description	there are 6 pieces of this product, each containing 0.5mL, frozen
species	Fungal kingdom, Aspergillus, Aspergillus flavus
traceability	Henan industrial microbial strain engineering technology research center & larr;CGMCC3.4410
preparation method	inoculation and culture, collection of hyphae and spores, liquid exchange suspension, 80 ℃ water bath inactivation for 1 hour
other ingredients	PBS, stabilizer
store	-80℃	validity period	2 years
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Quality inspection

project	standard	confirm test results
bacterial concentration	≥1.0×10⁷CFU/mL	6.2×10⁷CFU/mL
authentication	phenotypic characteristics, biochemical identification	Exactly consistent with Aspergillus flavus
authentication	gene sequencing	similarity rate 100%

4. Instructions

This reference product is not recommended for repeated freezing and thawing. Before use, take it out of the refrigerator, equilibrate to room temperature, mix well, and it is ready to use.

5. Matters needing attention

1. After receiving the goods, if any damage is found, please contact the center in time within 24 hours, and reissue it free of charge after confirmation;

2. This product uses heat inactivation method, which is likely to cause protein damage, and is only suitable for nucleic acid and other tests;

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833