BeNa Culture Collection
Culture medium | Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L. |
Growth conditions | Culture temperature 28 ℃; Culture time 5-10 days; Aerobic gas environment; |
Storage conditions | -80 ℃ |
Safety level | 2 |
application | Pathogenic nucleic acid detection kit cross-reaction, specificity test, and other nucleic acid related studies. |
Sharing mode | Public welfare sharing |
Aspergillus flavus nucleic acid reference certificate
This reference product was developed by Henan Industrial Microorganisms Engineering Technology Research Center and can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.
1. Product information
product name | Aspergillus flavus nucleic acid reference (heat inactivated) (strong positive) | ||
number | BNCC 361768 | batch | 220608 |
2. Product features
description | there are 6 pieces of this product, each containing 0.5mL, frozen | ||
species | Fungal kingdom, Aspergillus, Aspergillus flavus | ||
traceability | Henan industrial microbial strain engineering technology research center & larr;CGMCC3.4410 | ||
preparation method | inoculation and culture, collection of hyphae and spores, liquid exchange suspension, 80 ℃ water bath inactivation for 1 hour | ||
other ingredients | PBS, stabilizer | ||
store | -80℃ | validity period | 2 years |
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use |
3. Quality inspection
project | standard | confirm test results |
bacterial concentration | ≥1.0×107CFU/mL | 6.2×107CFU/mL |
authentication | phenotypic characteristics, biochemical identification | Exactly consistent with Aspergillus flavus |
gene sequencing | similarity rate 100% |
4. Instructions
This reference product is not recommended for repeated freezing and thawing. Before use, take it out of the refrigerator, equilibrate to room temperature, mix well, and it is ready to use.
5. Matters needing attention
1. After receiving the goods, if any damage is found, please contact the center in time within 24 hours, and reissue it free of charge after confirmation;
2. This product uses heat inactivation method, which is likely to cause protein damage, and is only suitable for nucleic acid and other tests;
Henan Engineering Research Center of Industrial Microbiology
website: www.bncc .org .cn tel: 400-6699-833