Lactobacillus casei nucleic acid reference material (Heat inactivated) (Strongly positive)|BNCC361727 |BNCC

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Lactobacillus casei nucleic acid reference material (Heat inactivated) (Strongly positive)

Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Storage conditions	-80 ℃
Safety level	0
application	This product is a heat-inactivated strong positive reference for the evaluation of Lactobacillus casei nucleic acid detection reagents, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing

Lactobacillus casei nucleic acid reference certificate

1. Product information

Sample name: Lactobacillus casei nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361727

Sample batch: 220602

2. Product features

Description	Packaged in a 1.5mL cryovial, containing 0.5mL nucleic acid reference material, in liquid state
Traceability	ATCC334 Lactobacillus casei
Preparation method	Inoculated with MRS liquid medium, collected by centrifugation, suspended in PBS, and inactivated in a water bath at 80 °C for 1 h
Concentration standard	≥1.0×10⁸CFU/mL	result	5.3×10⁸CFU/mL
Authentication	Colony morphology consistent with Lactobacillus casei	result	match
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference material at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. Nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833