Neisseria meningitidis nucleic acid reference material (Heat inactivated) (Strongly positive)|BNCC361726 |BNCC

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Neisseria meningitidis nucleic acid reference material (Heat inactivated) (Strongly positive)

Culture medium	Columbia agar chocolate plate (English name: CA-C): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 80-90 ℃, add 5% sterile defibrated sheep blood, shake well and pour the plate to cool for later use.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	37 ℃;18-24h;5% CO2 + 95% air
Storage conditions	-80 ℃
Safety level	2
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of Neisseria meningitidis nucleic acid detection reagent for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Neisseria meningitidis nucleic acid reference certificate

1. Product information

Sample name: Neisseria meningitidis nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361726

Sample batch: 220602

2. Product features

Description	Packaged in a 1.5mL cryovial, containing 0.5mL nucleic acid reference material, in liquid state
Traceability	ATCC13077 Neisseria meningitidis
Preparation method	Inoculate yeast extract wort medium, wash with PBS, inactivate in 80°C water bath for 1h
Concentration standard	≥1.0×10⁸CFU/mL	result	1.6×10⁸CFU/mL
Authentication	Colony morphology consistent with Neisseria meningitidis	result	match
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference material at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. Nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833