Bordetella pertussis nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361658 |BNCC

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Bordetella pertussis nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃;2-3 days; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of the nucleic acid detection reagent of Bordetella pertussis for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Bordetella pertussis nucleic acid reference certificate

1. Product information

sample name: bacillus pertussis nucleic acid reference (heat inactivated) (strongly positive)

Sample number: 361658

Sample batch: 220524

2. Product features

description	1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, in liquid state
traceability	ATCC8467 Bordetella pertussis
preparation method	inoculation of BHI + serum medium, PBS suspension, 80 ℃ water bath inactivation for 60min
concentration standard	≥1.0×10⁶CFU/mL	result	1.6×10⁶CFU/mL
authentication	colony morphology is consistent with bacillus pertussis	result	match
* Note: If you have any questions about reference items, please contact BNCC for help

3. Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4.Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be treated according to infectious substances. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage: -80℃, valid for 2 years; transport conditions: transport on dry ice.

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833