Bordetella bronchisptica nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361599 |BNCC

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Bordetella bronchisptica nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃;18-24 culture time hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
application	Research on Reagents for Detection of Pathogen Nucleic Acid
Sharing mode	Public welfare sharing

Boweria bronchitis nucleic acid reference certificate

1. product information

Sample name: Boweria bronchitis nucleic acid reference (heat inactivated) (strong positive)

Sample number: 361599

Sample batch: 220627

2. product features

description		1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, is transparent liquid
traceability		ATCC 4617 bout of bronchitis
preparation method		inoculation of NB liquid culture medium, centrifugal collection, PBS suspension, 80 ℃ water bath inactivation for 1h
concentration value	≥1.0×10⁸CFU/mL	reference value	1.85×10⁹CFU/mL
applicable method		Relevant research suitable for pathogen nucleic acid detection reagents
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4. notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be treated according to infectious substances. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. preservation and transportation

Long-term storage: -80℃, valid for 2 years; transport conditions: transport on dry ice.

Henan Engineering Research Center of Industrial Microbiology

website: www.bncc .org .cn tel: 400-6699-833