BeNa Culture Collection

Proteus vulgaris nucleic acid reference (Heat inactivated) (Strongly positive)-BNCC
  • Proteus vulgaris nucleic acid reference (Heat inactivated) (Strongly positive)-BNCC
  • Proteus vulgaris nucleic acid reference (Heat inactivated) (Strongly positive)-BNCC

Proteus vulgaris nucleic acid reference (Heat inactivated) (Strongly positive)

  • Price: $3143
  • number:BNCC361594
  • Packing:Frozen storage tube; Concentration ≥ 10 ^ 6CFU/mL;6 tubes/box
Essential Information Certificate Related Products
Proteus vulgaris nucleic acid reference (Heat inactivated) (Strongly positive)
Culture medium Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment;
Storage conditions -80 ℃
Safety level 0
application Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of common Proteus nucleic acid detection reagents for early diagnosis and quality control of infection.
Sharing mode Public welfare sharing

Common Proteus nucleic acid reference certificate

1. Product information

Sample name: Common Proteus nucleic acid reference (heat inactivated)

Sample number: 361594

sample batch: 220523

2. Product features

Description Packaged in a 1.5mL cryovial containing 0.5mL nucleic acid reference material in a  liquid state
Preparation method Inoculate nutrient broth medium, suspend in PBS, inactivate in 80°C water bath for 30min
concentration standard ≥106CFU/mL Reference value 4.0×106CFU/mL
authentication The colony morphology is consistent with that of Proteus vulgaris results match
*Note: If you have any questions about the reference materials, please contact our center (BNCC) for assistance before use

3. Instructions 

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4.Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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