Natioanl standard for Escherichia coli DNA content|BNCC361586 |BNCC

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Natioanl standard for Escherichia coli DNA content

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. Take out the standard and balance it to room temperature; 2, fully mixed after a short centrifuge, should try to avoid bubbles.
Growth conditions	37 ℃;18-24 hours; Aerobic
Storage conditions	-20 ℃
Safety level	1
application	It is suitable for quantitative detection of DNA content of Escherichia coli.
Sharing mode	Public welfare sharing

National standard quality certificate for determination of DNA content of Escherichia coli

1. product information

Sample name: e. coli DNA content determination national standard

Sample number: BNCC 361586

sample batch: 220517

2. product features

description	the appearance is transparent liquid, 1.5mL frozen storage tube packaging, each 100μL; Positive quality control 1 tube (25μL); Negative quality control 1 tube (25μL)
traceability	ATCC25922 Escherichia coli
attribute value	98.5μg/mL
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	Suitable for quantitative detection of Escherichia coli DNA content
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. product use

1. Take out the standard and equilibrate to room temperature;

2. After fully mixing, centrifuge briefly to avoid the generation of air bubbles.

4. notes

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This standard is only suitable for the detection of Escherichia coli DNA content, not for other purposes;

3. The research materials should be used as soon as possible after freezing and thawing, and can be stored at -20°C or below, but avoid repeated freezing and thawing more than 3 times;

4. The research materials should be treated as infectious substances during use, and the residual samples should be sterilized by high temperature and high pressure and discarded after use;

5. If there is any inconsistency in quality and quantity, the relevant compensation is limited to the product itself and does not involve any other losses.

5. preservation and transportation

Long-term storage: -20℃, valid for 1 year; transport conditions: transport on dry ice.

Henan Engineering Research Center of Industrial Microbiology

Website: www.bncc .org .cn Tel: 400-6699-833

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