BeNa Culture Collection
Culture medium | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | Before use, the nucleic acid reference is dissolved on ice, slightly mixed and briefly centrifuged and placed on ice for later use. |
Growth conditions | 30 ℃,18-24h, aerobic |
Storage conditions | -20 ℃, cold storage |
Safety level | 1 |
Separation substrate | Manganese oxide crusts from pipe |
application | It is used for the confirmation and evaluation of relevant measurement methods in the experimental analysis of Pseudomonas putida nucleic acid detection, and the quality control of the analysis process. |
Sharing mode | Public welfare sharing |
Pseudomonas putida nucleic acid standard quality control certificate
1. Product information
Sample name: Pseudomonas putida nucleic acid standard quality control
Sample number: 361458
Sample batch: 220505
2. Product features
Reagent composition | 1.5mL frozen storage tube package, containing 100μL nucleic acid quality control product, transparent liquid state; Positive quality control 1 tube (25μL); Negative quality control 1 tube (25μL) |
traceability | ATCC23483 |
packing specifications | 100μL /branch |
fixed value method | qPCR |
reference value | 105~106copies/μL |
applicable fields | suitable for confirmation and evaluation of relevant measurement methods and quality control in experimental analysis of nucleic acid detection of Pseudomonas putida |
save and transport | -20 ℃, valid for 1 year, dry ice transportation |
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use |
3. Instruction
Before use, dissolve the nucleic acid reference material on ice, mix gently and centrifuge briefly, then place on ice for later use
4. Notes
1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt;
2. This nucleic acid quality control product should be used as soon as possible after freezing and thawing, and can be stored at -20°C or below for a long time, but avoid repeated freezing and thawing more than 3 times;
3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination.
Henan Engineering Research Center of Industrial Microbiology
Website: www.bncc .org .cn Tel: 400-6699-833