Aspergillus fumigatus nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361400 |BNCC

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Aspergillus fumigatus nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Comprehensive PDA agar (English name: CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.
Subculture procedure	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	-80 ℃
Safety level	2
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product of Aspergillus fumigatus nucleic acid detection reagent is evaluated with heat inactivation strong positive reference product for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Aspergillus fumigatus nucleic acid reference product certificate

1. Product information

Sample name: Aspergillus fumigatus nucleic acid reference substance (heat inactivated) (strong positive)

Sample number: 361400

Sample batch: 220509

2. Product features

Description	Packaged in a 1.5mL cryovial, containing 0.5mL nucleic acid reference material, in liquid state
Traceability	ATCCMYA-3627 Aspergillus fumigatus
Preparation method	Inoculated on BCYE agar medium, eluted with PBS, and inactivated in a water bath at 80°C for 1h
Concentration standard	≥1.0×10⁷CFU/mL	results	6.3×10⁷CFU/mL
Authentication	Colony morphology consistent with Aspergillus fumigatus	results	match
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference material at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. Nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833