BeNa Culture Collection
Safety level | 0 |
Sharing mode | Public welfare sharing |
Salmonella nucleic acid test kit certificate
1. Product information
Sample name: salmonella nucleic acid detection kit (fluorescent PCR method)
Sample number: 361345
Sample batch: 220420
2. Product features
Reagent composition | qPCR mixed reaction solution 1 tube (525μL); Positive quality control 1 tube (100μL); Negative quality control 1 tube (100μL) |
packing specifications | 25 T/box |
sensitivity | 1.09copies/μL |
applicable fields | suitable for auxiliary diagnosis of salmonella infection in water-like feces, suspected contaminated water, food and other samples |
save and transport | -20 ℃, valid for 1 year, dry ice transportation |
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use |
3. Instruction
1. Experimental preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, and place on ice for later use;
2. Sample processing: sample processing according to "T/SDAQI 043-2021 Salmonella Inspection";
3. Reagent preparation: experimental reaction system (25 μL), 21 μL qPCR mixed reaction solution, 4 μL template;
4. Reaction conditions: 95°C 3min 1cycle; 94°C 10s, 50°C 30s 40cycle;
5. Fluorescence channel: HEX;
4. Result judgment
1. The sample has a typical amplification curve, and the Ct value is less than or equal to 35;
2. The sample has no amplification curve, and the Ct value is ≥40 or not detected;
3. If the t value of the sample is >35 and <40, it needs to be re-measured. Retest results Ct value ≥ 40 is negative, Ct value < 40 is positive.
5. Preservation and transportation
1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;
2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;
3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;
Henan Engineering Research Center of Industrial Microbiology
Website: www.bncc .org .cn Tel: 400-6699-833