BeNa Culture Collection
|Culture medium||Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.|
|Subculture procedure||Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 20min, then opened in the biosafety cabinet, 3-4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample with a total concentration of 100mL and a concentration of 10-100MPN/100mL.|
|Growth conditions||Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment|
|Storage conditions||-20 ℃, kept away from light|
|application||Quality control and personnel assessment of coliform count in food. Reference Standard "GB4789.3-2016 Food Safety National Standard Food Microbiological Detection Coliform Count" MPN Counting Method.|
|Sharing mode||Public welfare sharing|
Standard quality control sample certificate for coliform
Sample name: Coliform standard control sample (MPN count method)
Sample number: 360590
sample batch: 220105
2. Product features:
|Description||Appearance is white powder (lyophilized powder), containing protective agent, packaged in 7mL vial|
|Traceability||ATCC 25922 Escherichia coli, ATCC 13048 Enterobacter aerogenes CICC10404 Citrobacter freundii, ATCC6538 Staphylococcus aureus|
|Uniformity and stability||Random sampling, the results are uniform; long-term stability test, the stability is qualified|
|Applicable method||《GB4789.3-2016 National Food Safety Standard - Food Microbiological Testing - Coliform Counting》|
|*Note: If you have any questions about the quality control samples, please contact our center (BNCC) for help before use|
The samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 20 minutes, then open it in a biological safety cabinet, immediately add 3-4 mL of sterile water, dissolve it, suck it out and put it into a sterile bottle, and repeatedly wash the inner wall of the vial with the remaining sterile water. Transfer to the above sterile bottle, which is the standard quality control sample, a total of 100mL, and the concentration is 10~100MPN/100mL.
1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.
2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.
3. This product contains pathogenic microorganisms. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them.
5. Storage and transport
long-term storage: -20 ℃, valid for 1 year; Transportation conditions: ice bag transportation, the time should not exceed 10 days.
Henan Engineering Technology Research Center of Industrial Microbial Strain
website: www.bncc .org .cn; Tel: 400-6699-833