Standard quality control samples of Escherichia coli in drinking water (multi-tube fermentation method)|BNCC360415 |BNCC

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Standard quality control samples of Escherichia coli in drinking water (multi-tube fermentation method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above sterile bottle, which is a standard quality control sample with a total concentration of 100mL and a concentration of 100-2000MPN/100mL.
Growth conditions	37°C, aerobic, 18-24h
Storage conditions	-20 ℃, kept away from light
Safety level	2
application	Specific use: Quality control samples of Escherichia coli in drinking water (multi-tube fermentation method) Applicable Standard: GB/T5750.12-2006 "Microbiological Indicators of Standard Test Methods for Drinking Water"
Sharing mode	Public welfare sharing

Standard quality control sample certificate for Escherichia coli in drinking water

1. Product information

Sample name: Standard quality control sample of Escherichia coli in drinking water (multi-tube fermentation method)

Sample number: 360415

sample batch: 211206

2. Product features

Description	Appearance is white powder (lyophilized powder), containing protective agent, packaged in 7mL vial
Traceability	ATCC 25922 Escherichia coli, ATCC 13048 Enterobacter aerogenes ,ATCC6538 Staphylococcus aureus
Concentration value	100-2000MPN/100mL
Reference value	540MPN/100mL
Uniformity and stability	Random sampling, the results are uniform; long-term stability test, the stability is qualified
Applicable method	《GB/T 5750.12-2006 Standard Test Methods for Drinking Water, Microbiological Indicators》
*Note: If you have any questions about the quality control samples, please contact our center (BNCC) for help before use

3. Instructions

Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, place the quality control sample at room temperature for 30 minutes, then open it in a biological safety cabinet, immediately add 3-4 mL of sterile water, dissolve it, suck it out and put it into a sterile bottle, and repeatedly wash the inner wall of the vial with the remaining sterile water. Transfer to the above sterile bottle, which is the standard quality control sample, a total of 100mL, and the concentration is 100-2000MPN/L.

4. Notes:

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.

2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.

3. This product contains pathogenic microorganisms. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them.

5. Saving and shipping:

long-term storage: -20 ℃, valid for 1 year; Transportation conditions: ice bag transportation, the time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn; Tel: 400-6699-833