BeNa Culture Collection
Culture medium | Tryptone soybean medium (English name: TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min. |
Subculture procedure | (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry; |
Growth conditions | 30 ℃;18-24h; Aerobic; |
Storage conditions | -20 ℃ |
morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), bacilli, purity: pure |
Sharing mode | Public welfare sharing |
Bacillus subtilis subsp.
Strain number: BNCC 361421 /ATCC6633
Product format: quantitative bacteria tablets
Number of viable bacteria:(4.7±0.3)×107CFU/bottle
Product batch: 220428
Valid until: 2023.04.27
Date of inspection: 2022.04.27
Sampling ratio:≥2%
Report number: RS22293727-1
Biosafety level: 1, handle in ultra-clear table or safety cabinet
items | description | results |
stain microscopy | Gram-negative, positive, cell morphology observed under microscope | G + cocci |
colony morphology | Observe the morphology of single colonies | golden yellow, raised, bright, moist |
purity | Non-selective culture medium, colony observation and microscopy |
No contaminated bacterial |
Reference value of live bacteria | Non-selective media plates, viable count | (4.7±0.3)×107CFU |
Phenotypic and Genotypic Analysis | Phenotypic testing, gene sequencing | qualified |
test conclusion | After the BNCC quality control inspection system, the product is qualified |
Product list: quantitative strains 1 bottle, 1mL lysate 1 bottle, 1 strain certificate;
Validity period: unopened, below -20 ℃, 12 months.
Usage:
1. Open the strain and lysate according to the arrow indication of the vial cap;
2. Pour the bacterial flakes into the dissolving solution, cover with a rubber stopper, and shake to dissolve;
3. After fully dissolved, it is (4.7±0.3)×107CFU/mL, and it can be drawn in proportion according to the test requirements;
4. Please use up immediately after opening and do not store;
Notes:
① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.
②Used germ-carrying consumables should be discarded after autoclaving.