BeNa Culture Collection
|Culture medium||Tryptone soybean medium (English name: TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.|
|Subculture procedure||(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;|
|Growth conditions||30 ℃;18-24h; Aerobic;|
|Storage conditions||-20 ℃|
|morphology||The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), bacilli, purity: pure|
|Sharing mode||Public welfare sharing|
Bacillus subtilis subsp.
Strain number: BNCC 361420 /ATCC6633
Product format: quantitative bacteria tablets
Number of viable bacteria: (5.4±0.5)×106CFU/bottle
Product batch: 220428
Valid until: 2023.04.27
Date of inspection: 2022.04.27
Report number: RS22293727-1
Biosafety level: 1, handle in ultra-clear table or safety cabinet
|stain microscopy||Gram-negative, positive, cell morphology observed under microscope||G + cocci|
|colony morphology||Observe the morphology of single colonies||off-white, flat, rough, rounded or irregular edges|
|purity||Non-selective culture medium, colony observation and microscopy||
No contaminated bacterial
|Reference value of live bacteria||Non-selective media plates, viable count||5.4×106CFU/bottle|
|Phenotypic and Genotypic Analysis||Phenotypic testing, gene sequencing||qualified|
|test conclusion||After the BNCC quality control inspection system, the product is qualified|
Product list: (2.0±0.5)×10^7CFU quantitative strains 1 bottle, 1mL lysate 1 bottle, 1 strain certificate;
Validity period: unopened, below -20 ℃, 24 months.
1. Open the strain and lysate according to the arrow indication of the vial cap;
2. Pour the bacterial flakes into the dissolving solution, cover with a rubber stopper, and shake to dissolve;
3. After fully dissolved, it is (5.4±0.5)×106CFU/mL, and it can be drawn in proportion according to the test requirements;
4. Please use up immediately after opening and do not store;
① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.
②Used germ-carrying consumables should be discarded after autoclaving.