Candida albicans quantitative strain|BNCC359899 |BNCC

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Candida albicans

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After full dissolution, it is 50 ~ 100CFU/ml, and it is absorbed in proportion according to the requirements; 4. Please use up immediately after opening, and do not store
Growth conditions	28 ℃;24-48h; Aerobic;
Growth characteristics	Cells round or oval, polygonal budding.
Storage conditions	-80 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, milky white on the front, milky white on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, G + (blue-purple), cocci, purity: pure
application	Research; Analytical testing. "Technical Specifications for Disinfection" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Therapeutics, "GB 4789.15-2010 Mold and Yeast Count" Positive Control Strain, 2015 Edition Chinese Pharmacopoeia Quality Control Strain.
Sharing mode	Public welfare sharing

Candida albicans

Strain number: BNCC 359899 / CMCC(F)98001

Product format: quantitative bacteria tablets

Number of viable bacteria: 50~ 100CFU/bottle

Product batch: 22011201

Valid until: 2023.01. 1 1

Date of inspection: 2022.01. 13

Sampling ratio:≥2%

Report number: RS22359899- 1

Biosafety level: 1 , handle in ultra-clear table or safety cabinet

items	description	results
stain microscopy	Gram-negative, positive, cell morphology observed under microscope	G + cocci
colony morphology	Observe the morphology of single colonies	Milky white, moist, raised, with neat edges
purity	Non-selective culture medium, colony observation and microscopy	No contaminated bacterial
Reference value of live bacteria	Non-selective media plates, viable count	94CFU/bottle
Phenotypic and Genotypic Analysis	Phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system, the product is qualified

Product list: one bottle of 50~ 100CFU/bottle quantitative strains , 1mL lysate 1 bottle, 1 strain certificate;

Validity period: unopened, below -20 ℃, 12 months.

Usage:

1. Open the strain and lysate according to the arrow indication of the vial cap;

2. Pour the bacterial flakes into the dissolving solution, cover with a rubber stopper, and shake to dissolve;

3. After fully dissolved, it is 50~ 100CFU/mL, and it can be drawn in proportion according to the test requirements;

4. Please use up immediately after opening and do not store;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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