Quantitative strain of Enterobacter cloacae|BNCC354948 |BNCC

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Quantitative strain of Enterobacter cloacae

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	-20 ℃
morphology	Pale yellow, raised, moist, bright, G-bacilli
Sharing mode	Public welfare sharing

Enterobacter cloacae

strain number:BNCC 354948

Name: Enterobacter cloacae

Product format: quantitative bacteria tablets Number of viable bacteria: 2.4 × 10^6CFU/bottle

Product batch: 220317 Valid until: 2024.03. 16

Inspection date: 2022.03. 18 Sampling ratio: ≥2%

Report number: RS22354948- 1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

project	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	pale yellow, raised, moist, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	2.4 × 10^6CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

product list: One bottle of 2.4 × 10^6CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;

shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is 2.4 × 10^6CFU/bottle, which can be sucked in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.