BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry; |
Growth conditions | 30 ℃;18-24h; Aerobic; |
Storage conditions | -20 ℃ |
morphology | Pale yellow, raised, moist, bright, G-bacilli |
Sharing mode | Public welfare sharing |
Enterobacter cloacae
strain number:BNCC 354948
Name: Enterobacter cloacae
Product format: quantitative bacteria tablets Number of viable bacteria: 2.4 × 10^6CFU/bottle
Product batch: 220317 Valid until: 2024.03. 16
Inspection date: 2022.03. 18 Sampling ratio: ≥2%
Report number: RS22354948- 1 Biosafety level: 1, handle in ultra-clear table or safety cabinet
project | Inspection description | results |
stain microscopy | gram negative and positive, cell morphology observed under microscope | G-bacilli |
colony morphology | observation of single colony morphology | pale yellow, raised, moist, bright |
Purity | non-selective medium culture, colony observation and microscopic examination | No contaminated bacterial |
Reference value of live bacteria | Non-selective medium plate, viable count | 2.4 × 10^6CFU/bottle |
Phenotypic and Genotypic Analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | After the BNCC quality control inspection system quality inspection, the product is qualified |
product list: One bottle of 2.4 × 10^6CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;
shelf life: unopened, below -20 ℃, 24 months;
Usage:
1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;
2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;
3. After fully dissolved, it is 2.4 × 10^6CFU/bottle, which can be sucked in proportion according to the test requirements;
4. Please use up immediately after opening, and do not store it;
notes:
① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.
② Used bacteria-carrying consumables, please autoclave and discard them.