BeNa Culture Collection

Staphylococcus aureus subsp. aureus Rosenbach-BNCC
Staphylococcus aureus subsp. aureus Rosenbach-BNCC
Staphylococcus aureus subsp. aureus Rosenbach-BNCC
  • BNCC
  • Staphylococcus aureus subsp. aureus Rosenbach-BNCC
  • Staphylococcus aureus subsp. aureus Rosenbach-BNCC
  • Staphylococcus aureus subsp. aureus Rosenbach-BNCC

Staphylococcus aureus subsp. aureus Rosenbach

  • Price: $86
  • number:BNCC353548
  • Packing:penicillin bottle; 500 ~ 1000CFU/bottle
  • Form:
    Purity: pure
Essential Information Certificate Related Products
Staphylococcus aureus subsp. aureus Rosenbach
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions 37°C, aerobic
Storage conditions -20 ℃
morphology Purity: pure
Separation substrate Human lesion
Sharing mode Public welfare sharing

Staphylococcus aureus

No.:353548 / ATCC 6538

Product format: quantitative bacteria tablets

Number of live bacteria : 0.5-1.0x10 3CFU/bottle

Product batch : 200427

Valid until : 2021.04.27

Date of inspection : 2020.04.28

Sampling ratio : ≥2%

Report number : RS20353548-1

Biosafety level : 2 , handle in safety cabinet

item description test results
stain microscopy Gram-positive, observe cell morphology under microscope G + cocci, in the shape of single ball, hammer ball and grape string
colony morphology observation of single colony morphology golden yellow, raised, bright, round
purity non-selective medium culture, colony observation and microscopic examination No bacteria
Reference value of live bacteria non-selective medium plate, live bacteria count 680CFU/bottle
phenotype and genotype analysis phenotypic testing, gene sequencing qualified
test conclusion after quality inspection by BNCC quality control testing system, the product is qualified  

Product list :0.5-1.0x10 3CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period : unopened, below -20 ℃, 12 months;

Handling procedure:

1  Open the penicillin-bottle according to the arrow indication on the vial cap;
2  Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3  It will be 0.5-1.0x10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4  The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2  Waste generated from the handling process should be discarded after high-pressure sterilization

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