Bacillus subtilis|353756 |BNCC

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Bacillus spizizenii (Nakamura et al.) Dunlap et al.

Subculture procedure	1 Prepare one sterile liquid test tube (containing 5-10mL of culture medium) and two 90mm plates; two After disinfecting the wall of the penicillin bottle, in a sterile environment, open the aluminum cap and rubber stopper in sequence, draw about 0.5mL of liquid culture medium into the bottle, gently blow and dissolve the bacterial slices; three After the bacterial tablets are fully dissolved, aspirate all the dissolved solution and mix it evenly in the liquid test tube; four Draw the mixed solution from the test tube liquid and apply it to the Agar plate at a rate of 200 μ L per Agar plate; five Place all test tubes and plates under the specified conditions for cultivation, and prohibit sealing and wrapping the plates. After 18-24 hours, observe that the culture medium is significantly turbid and that the Agar plate colonies or bacterial moss are growing; six If the strain has not grown, it should be further cultured for 72 hours, and after cultivation, it should be used according to the specific experimental requirements.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough on the surface, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Bacillus subtilis subsp.

No. : 353756

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level : 1

Validity :period 5 years

Use : pharmacopoeia, national standard and other control quality control strains

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium : nutrition gravy medium beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2ml of the solution, distribute it well in 200ml/plate;
(5)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grows well.
(6) If it does not grow in 18-24 hours, culture is continued until 72 hours is reached. And the strains can be used when they grow

Growth conditions temperature: 37 ℃ ; Gas environment: aerobic ; Time: 18-24h

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