Bacillus subtilis|353756 |BNCC

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Bacillus spizizenii (Nakamura et al.) Dunlap et al.

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	1 Prepare one sterile liquid test tube (containing 5-10mL of culture medium) and two 90mm plates; two After disinfecting the wall of the penicillin bottle, in a sterile environment, open the aluminum cap and rubber stopper in sequence, draw about 0.5mL of liquid culture medium into the bottle, gently blow and dissolve the bacterial slices; three After the bacterial tablets are fully dissolved, aspirate all the dissolved solution and mix it evenly in the liquid test tube; four Draw the mixed solution from the test tube liquid and apply it to the Agar plate at a rate of 200 μ L per Agar plate; five Place all test tubes and plates under the specified conditions for cultivation, and prohibit sealing and wrapping the plates. After 18-24 hours, observe that the culture medium is significantly turbid and that the Agar plate colonies or bacterial moss are growing; six If the strain has not grown, it should be further cultured for 72 hours, and after cultivation, it should be used according to the specific experimental requirements.
Storage conditions	-20 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough on the surface, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing