BeNa Culture Collection

Bacillus subtilis-BNCC
Bacillus subtilis-BNCC
  • BNCC
  • Bacillus subtilis-BNCC
  • Bacillus subtilis-BNCC

Bacillus subtilis

  • Price: $ 172
  • number:BNCC361060
  • Form:
    The colony diameter is 2-4mm, round, irregular edges, opaque, gray-white on the front, convex in the middle, rough surface, gray surface, moist texture, easy to pick up, G + (blue-purple), bacilli
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Bacillus subtilis
Culture medium Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic
Storage conditions 2-8 ℃
morphology The colony diameter is 2-4mm, round, irregular edges, opaque, gray-white on the front, convex in the middle, rough surface, gray surface, moist texture, easy to pick up, G + (blue-purple), bacilli
Sharing mode Public welfare sharing

Bacillus subtilis

Storage conditions: 2~8 ℃

No. 361060

Product format: freeze dried, 200ul 

Validity period: 6 years

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min.  

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability

good viability, in 18h, plate bacteria layer is obvious;

colony is typical on the streaked plate

colony morphology:

Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque

Color: off-white bulge: middle bulge surface: coarse and super gray texture: moist and easy to stir

Conclusion: good viability, no abnormal colony morphology, qualified
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