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Streptococcus pneumoniae
Storage conditions: 2~8 ℃
No. 360203
Product format: freeze dried,200ul
Validity period: 6 years
Biosafety level: 2, handle in safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:37 ℃,5% CO2, Columbia blood plate (ready-to-use type),18-24h.
Recovery steps:
①Prepare 1-2 of above mentioned plates;
②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
④Put the plates under the above culture conditions for cultivation 18-24h.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
Colony morphology:
(pictured above)
Size: 0.5-1mm Shape: Round Edge: Irregular Transparency: Opaque Color: Gray Yellow
Protuberance: Middle convex surface: bright and smooth texture: moist and viscous