Clostridium sporogenes|BNCC351961 |BNCC

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Clostridium sporogenes

Culture medium	Liquid thioglycolate medium (FT): 15.0g of caseptone (trypsin hydrolysis), 5.0g of yeast extract, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 2.5g of sodium chloride, 0.001g of azure, 0.75g of agar, 1.0L of distilled water and 7.1±0.2 of pH value. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;2-3 days; Anaerobic;
Growth characteristics	G +, cell straight rod-shaped, 0.73 × 1.46~3.33 μm. Mostly a single arrangement. The spores are oval, subterminal, and the spores are enlarged. After the formation of spores, the bacteria quickly decomposed, leaving only spores.
Storage conditions	-20 ℃
morphology	G + (blue-purple), bacilli, purity: pure
application	Research; Analytical testing. Teaching and research. "Technical Specifications for Disinfection" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Therapeutics, "GB/T 4789.12-2003 Clostridium botulinum and Botulinum Toxin Test" Positive Control Strain, Quality Control Strain of Chinese Pharmacopoeia, 2015 Edition.
Sharing mode	Public welfare sharing

Clostridium sporogenes

No. : 351961

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level : 2

Validity period: 5 years

Use : pharmacopoeia, national standard and other control quality control strains

Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium : thioglycolate medium peptone 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, azure 0.001g, sodium chloride 2.5g,pH7.1±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium.
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 48-72 hours, and this indicates the bacterial grows well.
(5)If it does not grow in 18-24 hours, culture is continued until 96 hours is reached. And the strains can be used when they grow

Growth conditions temperature: 37 ℃ Gas environment: anaerobic Time: 48-72h