Brucella sheep|BNCC340736 |BNCC

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Brucella sheep

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,;24-48;5% CO2;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Milk White Protuberance: Middle Protuberance Surface: Bright and Smooth Texture: Wet and Easy to Stir
Separation substrate	Animal Organization, Australia
application	Study on zoonotic diseases
Sharing mode	Public welfare sharing

Brucella ovis Brucella

Storage conditions : 2~8 ℃

No. : 340736

Product format: freeze dried, 200ul, or flat plate 1

Validity period : freeze-dried tube for 24 months, flat plate for 7 days

Biosafety level : 2,handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Agar plates can be used directly. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:
(1)Prepare 1-2 pieces of Blood agar plates;

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well in 200ml/plate;

(4)Put the agar plates under the above culture conditions for cultivation 24-48 hours.

Recovery/subculture: 37℃，5%CO2，Columbia Blood agar plate

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 24 hours Columbia blood strain layer is obvious
colony morphology	size: smaller color: off-white shape: round edge: neat edge dry and wet: moist and smooth: smooth transparency: opaque uplift: bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified