|Culture medium||Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.|
|Subculture procedure||(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.|
|Growth conditions||28 ℃;2-3 days; Aerobic|
|Storage conditions||2-8 ℃|
|morphology||G + bacillus size: 1-2mm shape: round edge: irregular transparency: opaque color: light yellow uplift: middle convex surface: rough texture: dry and difficult to pick|
|application||Type strain, producing the defense line bacterin X complex|
|Sharing mode||Public welfare sharing|
2. BNCC No.:337591
3. Biosafety level: 4
Storage of freezed dried ampoule and agar slant at 2°C to 8°C
3. Growth conditions
1. ISP-2 medium: yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 15.0g, distilled water 1.0 L,pH7.3. 121 ℃,15 minutes sterilization.
2. Atmosphere: aerobic
3. Temperature: 28 ℃
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.
2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.
3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.
4.Waste generated from the handling process should be discarded after high-pressure sterilization.