BeNa Culture Collection

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BNCC > Microbial strain > Chromobacterium purpurea
Chromobacterium purpurea-BNCC
Chromobacterium purpurea-BNCC
  • BNCC
  • Chromobacterium purpurea-BNCC
  • Chromobacterium purpurea-BNCC

Chromobacterium purpurea

  • Price： $ 172
  • number：BNCC337438
  • Form：
    Size: General Color: Purple Black, Metallic Gloss Shape: Round Edge: Neat Edge Dry and Wet: Wet and Smooth: Smooth Transparency: Opaque Protuberance: Bulge
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Chromobacterium purpurea
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;24-48h; Aerobic
Storage conditions 2-8 ℃
morphology Size: General Color: Purple Black, Metallic Gloss Shape: Round Edge: Neat Edge Dry and Wet: Wet and Smooth: Smooth Transparency: Opaque Protuberance: Bulge
Separation substrate Freshwater, Malaysia
Sharing mode Public welfare sharing

Chromobacterium violaceum

Storage conditions : 2~8 ℃

No. : 337438

Product format: 90mm flat

Validity period : Tablet 7 days

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :37°C, aerobic, nutrient agar/gravy medium (NA/NB). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.
(2)Open the plates with strains in biosafety cabinet, mark it,  then inoculate a fresh agar plate or test tube containing liquid medium 
(3)Place the test tube in plate shaker  at 140r/min;   sit the plates in incubator for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

                                              

item test results
viability good viability, in24h,NB bacterial fluid turbid; Marked NA strain layer is obvious
colony morphology size: general color: purple-black, metallic luster shape: round edge: neat edge dry and wet: moist and smooth: smooth transparency: opaque uplift: bulge
conclusion good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified
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