BeNa Culture Collection

Pseudozyma antarctica-BNCC
Pseudozyma antarctica-BNCC
  • BNCC
  • Pseudozyma antarctica-BNCC
  • Pseudozyma antarctica-BNCC

Pseudozyma antarctica

  • Price: $ 143
  • number:BNCC337339
  • Form:
    Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: White Protuberance: Middle Protuberance Surface: Rough Texture: Dry Hard
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Pseudozyma antarctica
Culture medium YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;48-72h; Aerobic
Storage conditions 2-8 ℃
morphology Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: White Protuberance: Middle Protuberance Surface: Rough Texture: Dry Hard
application Type strain. Production of heat-resistant, non-specific lipase
Sharing mode Public welfare sharing

Pseudozyma antarctica

Storage conditions : 2~8 ℃

No. : 337339

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃,YM, aerobic, 72h,YM medium: yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation,and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

                                     

item test results
viability good viability,in 72h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above) size: 2-4mm shape: round edge: irregular transparency: opaque color: white uplift: middle raised surface: rough texture: dry and hard
conclusion good viability, no abnormal colony morphology, qualified
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