BeNa Culture Collection

Lysinibacillus boronitolerans-BNCC
Lysinibacillus boronitolerans-BNCC
  • BNCC
  • Lysinibacillus boronitolerans-BNCC
  • Lysinibacillus boronitolerans-BNCC

Lysinibacillus boronitolerans

  • Price: $ 143
  • number:BNCC337152
  • Form:
    Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to pick
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Lysinibacillus boronitolerans
Culture medium Tryptone soybean medium (TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic
Storage conditions 2-8 ℃
morphology Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to pick
Separation substrate Soil, Japan
application Type strain
Sharing mode Public welfare sharing

Lysinibacillus boronitolerans

Storage conditions : 2~8 ℃

No. : 337152

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, aerobic, tryptone soybean medium, 18-24h. Tryptone soybean medium: tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability:

good viability, in 24 hours liquid medium become turbid, strain layer is obvious;

colony is typical on the streaked plate,

colony morphology: (above)

Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque

color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir

Conclusion: good viability, no abnormal colony morphology, qualified

 

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