Vibrio alginolyticus|BNCC337013 |BNCC

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Vibrio alginolyticus

Culture medium	2216E seawater agar (2216E): peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g,NaCl 19.45g,MgCl2 8.8g,Na2SO4 3.24g,CaCl2 1.8g,KCl 0.55g,NaHCO3 0.16g,NH4NO3 0.0016g,Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, agar 15.0g, distilled water 1000ml,pH 7.4-7.8. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;18-24h; Aerobic
Storage conditions	2~8 ℃
morphology	Size: 0.5-1mm shape: round edge: neat transparency: translucent Color: white bulge: middle bulge surface: bright texture: moist and easy to stir up
Separation substrate	Seawater
Sharing mode	Public welfare sharing

1.Description

1, BNCC No.:337013

2. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Marine Agar 2216: protein building 5.00g yeast powder 1.00g Fe(III) citrate 0.10g NaCl 19.45g anhydrous MgCl2 5.90g Na₂SO₄ 3.24g CaCl₂ 1.80g KCl 0.55g NaHCO3 0.16g KBr 0.08g SrCl₂ 34.00 mg H3BO3 22.00 mg Na-silicate 4.00 mg NaF 2.40 mg (NH4)NO3 1.60 mg Na2HPO4 8.00 mg distilled water 1000.00 ml pH 7.6±0.2. 121 ℃,15min.

2, Atmosphere:aerobic

3, Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.