BeNa Culture Collection

Escherichia cloacae-BNCC
Escherichia cloacae-BNCC
  • BNCC
  • Escherichia cloacae-BNCC
  • Escherichia cloacae-BNCC

Escherichia cloacae

  • Price: $ 86
  • number:BNCC336662
  • Form:
    The colony diameter is 2-4mm, round, with neat edges, opaque, light color, flat shape, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Escherichia cloacae
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic
Storage conditions 2-8 ℃
morphology The colony diameter is 2-4mm, round, with neat edges, opaque, light color, flat shape, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure
Sharing mode Public welfare sharing

Escherichia cloacae

Storage conditions : 2~8 ℃

No. : 336662

Product format: freeze dried, 200ul

Validityperiod : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :30°C, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                           

item test results
viability good viability, in 18-24h liquid medium become turbid,  obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology: (above) size: 2-4mm shape: round edge: neat transparency: opaque color: off-white uplift: middle raised surface: bright and smooth texture: moist and easy to pick up
conclusion good viability, no abnormal colony morphology, qualified
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