BeNa Culture Collection

Monascus purpureus Went-BNCC
  • BNCC
  • Monascus purpureus Went-BNCC

Monascus purpureus Went

  • Price: $ 86
  • number:BNCC336565
  • Form:
    Small filamentous fungus, well grown on wort agar medium, with purple-red colonies, raised in the middle of the colonies, with folds and radiation stripes.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Monascus purpureus Went
Culture medium Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;7-10 days; Aerobic;
Storage conditions 2-8 ℃
morphology Small filamentous fungus, well grown on wort agar medium, with purple-red colonies, raised in the middle of the colonies, with folds and radiation stripes.
application Production of red yeast rice, red pigment.
Sharing mode Public welfare sharing

Monascus purpureus Went

Storage conditions: 2~8 ℃

No. 336565

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level:  1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture: 28 ℃, aerobic, 10d, wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 2 wort agar plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                               

item test results
Viability: good viability, in 10 days plate colony is obvious
colony morphology: Small filamentous fungus, growing well on wort agar medium, with purple-red colonies, raised colonies in the middle, with folds and radiation stripes.
Conclusion: good viability, no abnormal colony morphology, completely consistent with the above figure, qualified
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