Azotobacter brown ball|BNCC192292 |BNCC

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Azotobacter brown ball

Culture medium	Nitrogen fixing medium (ABM):KH2PO4 0.2g,K2HPO4 0.8g,MgSO4 · 7H2O 0.2g,CaSO4 · 2H2O 0.1g,FeCl3 trace g,Na2MoO4 · 2H2O trace g, yeast extract 0.5g, mannitol 20.0g, agar 15.0g, distilled water 1.0L,pH 7.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;48-72h; Aerobic
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, golden yellow on the front, opaque, golden yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, purity: pure, G-(red), bacilli
Separation substrate	Soil
application	Research; Production. Nitrogen-fixing bacteria, bacterial fertilizers, microbial strains used in fertilizer production
Sharing mode	Public welfare sharing

1.Description

1. Name: Azotobacter chroococcum

2.BNCC No.:192292

3. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Medium name: nitrogen fixing medium

2. Medium components: yeast extract 0.5g, mannitol 20.0g,KH2PO4 0.2g,K2HPO4 0.8g,MgSO4.7H2O 0.2g,CaSO4·2H2O 0.1g;FeCl3 trace,Na2MoO4·2H2O trace, agar 15.0g, distilled water 1.0L,pH7.2.

3. Atmosphere: aerobic

4. Temperature: 25-28 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization