Aspergillus niger|BNCC186380 |BNCC

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Aspergillus niger

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days.; Aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread growth, black spore production, front black, dark, purity: pure
application	Research; Analytical testing. 2015 edition Chinese Pharmacopoeia Quality Control Strain, GB 4789.15-2010 Mold and Yeast Count Positive Control Strain. Spore production. 2020 edition of Pharmacopoeia-"General Principles 1100 Biological Inspection Method" for test strains for pharmacopoeia, culture medium "sensitivity inspection" for test strains, "method applicability test" for test strains, "counting medium applicability inspection and test sample counting method applicability test" for test strains, microbial counting "medium applicability test and method applicability test" for test strains, "Medium Applicability Test, Method Applicability Test, Bacteriostatic Efficacy Test" Test Strain GB 4789.15-2016 Mold and Yeast Count
Sharing mode	Public welfare sharing

Aspergillus niger

Storage conditions : 2~8 ℃

No. : 186380

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA agar, 5-7 days, integrated PDA culture medium: potato boiling solution 1.0L, glucose 20.0g,KH₂PO₄ 3.0g, MgSO₄·7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 5-7 days strain layer become obvious, colony is typical
colony morphology: (above)	filamentous fungi, with obvious colonies on integrated PDA agar medium, white hyphae, light yellowish brown on the reverse side, dense and long, hyphae Spread and grow, producing a large number of black spores.
Conclusion:	good viability, no abnormal colony morphology, qualified