BeNa Culture Collection
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Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Agar slant: growing culture, ready to use, Short term preservation, convenient access
| Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores. |
| Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
| Growth conditions | 30 ℃;1-2 days; Aerobic; |
| Storage conditions | 2-8 ℃ |
| morphology | The colony diameter is 0.5-1mm, round, with neat edges, opaque, yellow on the front, light color, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G + (blue-purple), cocci |
| Separation substrate | bottom mud |
| Sharing mode | Public welfare sharing |
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