BeNa Culture Collection
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Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Agar slant: growing culture, ready to use, Short term preservation, convenient access
| Culture medium | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores. |
| Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
| Growth conditions | 30 ℃;24-48h; Aerobic; |
| Growth characteristics | Gram-negative, microaerobic, nitrogen fixation |
| Storage conditions | 2-8 ℃ |
| morphology | Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: white bulge: middle bulge surface: bright and smooth texture: moist and viscous |
| Sharing mode | Public welfare sharing |
Azospirillum brasilense
Storage conditions: 2~8 ℃
No.: 139156
Product format: freeze dried,200ul
Validity period: 6 years
Biosafety level: 1, handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:30°C, aerobic, nitrogen-fixing medium, 18-24h. Nitrogen fixation medium: KH2PO4 0.2g,K2HPO4 0.8g,MgSO4.7 H2O 0.2g,CaSO4.2 H2O 0.1g,FeCl3 trace, Na2MoO4.2H2O trace, yeast extract 0.5g, mannitol 20.0g, agar 15.0g, distilled water 1.0 L,pH 7.2 sterilization.
Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a agar plate evenly.
(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability,in 24-48h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate. |
| colony morphology: (above) | size: 2-4mm shape: round edge: irregular transparency: opaque color: white uplift: middle convex surface: bright and smooth texture: moist and viscous |
| Conclusion | good viability, no abnormal colony morphology, qualified |
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