BeNa Culture Collection

Lactococcus lactis subsp. lactis-BNCC
Lactococcus lactis subsp. lactis-BNCC
  • BNCC
  • Lactococcus lactis subsp. lactis-BNCC
  • Lactococcus lactis subsp. lactis-BNCC

Lactococcus lactis subsp. lactis

  • Price: $ 215
  • number:BNCC134346
  • Form:
    Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: White Protuberance: Middle Bulge Surface: Bright and Smooth Texture: Wet and Viscous
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Lactococcus lactis subsp. lactis
Culture medium MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃,48-72h, micro aerobic
Storage conditions 2-8 ℃
morphology Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: White Protuberance: Middle Bulge Surface: Bright and Smooth Texture: Wet and Viscous
application Produces nisin
Sharing mode Public welfare sharing

Lactococcus lactis subsp. lactis

Storage conditions : 2~8 ℃

No. : 134346

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety  level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, micro aerobic, MRS medium, 48-72h. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation,and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

                          

item test result
viability: good viability, in 48-72h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.

Colony morphology:

(pictured above)

Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque

color: white uplift: middle convex surface: bright and smooth texture: moist and viscous

Conclusion: good viability, no abnormal colony morphology, qualified
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