Yersinia enterocolitis|108930 |BNCC

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Yersinia enterocolitica

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; Aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	Colony diameter: 0.5-1 mm Shape: Circular Edge: Entire Transparency: Opaque Color (front): Grayish-white Elevation: Umbonate Surface: Smooth and glossy Texture: Moist and easily picked up Gram stain: Negative (red) Cell morphology: Rod-shaped (bacillus) Purity: Pure
Sharing mode	Public welfare sharing