Sheep Brucella|340736 |BNCC

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Brucella ovis

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 24-48h；5%CO2；
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), short rod-shaped bacteria, purity: pure
Sharing mode	Public welfare sharing

Brucella ovis Brucella

Storage conditions : 2~8 ℃

No. : 340736

Product format: freeze dried, 200ul, or flat plate 1

Validity period : freeze-dried tube for 24 months, flat plate for 7 days

Biosafety level : 2,handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Agar plates can be used directly. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:
(1)Prepare 1-2 pieces of Blood agar plates;

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well in 200ml/plate;

(4)Put the agar plates under the above culture conditions for cultivation 24-48 hours.

Recovery/subculture: 37℃，5%CO2，Columbia Blood agar plate

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 24 hours Columbia blood strain layer is obvious
colony morphology	size: smaller color: off-white shape: round edge: neat edge dry and wet: moist and smooth: smooth transparency: opaque uplift: bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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