Escherichia coli plate counting standard substance|366383 |BNCC

Essential Information Related Products

Reference material of Escherichia coli Count

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃, aerobic
Subculture procedure	① positive quality control bacteria:; ② Negative quality control bacteria:; ③ Activate and enhance the bacteria according to the above quality control samples, so that the absorbance of the ten fold dilution solution of the two bacteria is within the range of 0.3~0.5; ④ Mix the two original bacterial solutions equally in volume, dilute them 25000 times, and then take 0.5mL of the final dilution solution and inject it into 10mL of protective agent (quantitative bacterial protective agent), mix well; ⑤ Divide the mixture into 0.4mL/penicillin bottles and pre freeze at -80 ° C for 20-24 hours. Remove and freeze overnight. The next day, seal and label the freeze-dried quality control product for storage;
Storage conditions	-20 ℃
Sharing mode	Public welfare sharing