Mycoplasma gallisepticum Edward and Kanarek|376418 |BNCC

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Mycoplasma gallisepticum Edward and Kanarek

Growth conditions	37°C, 95% Air, 5% CO₂
Subculture procedure	a. Open the vial according to the enclosed instructions.b. Rehydrate the entire pellet with approximately 0.25 mL of #243 broth. Aseptically transfer theentire contents to primary tube containing 2.25mL of #243 broth. Make serial dilutions bytransferring 0.25mL from the primary tube to second to third tube,etc.c. Plates may be inoculated to check colonial morphology. You can also spot each dilution on thesurface of Agar plate (4 or more/Agar plate) to determine the number of colony-forming units. However,not all strains do well on solid medium.d. Incubate broth tubes at 37°C,aerobic,and the plates at 37°C 5% cOz for 3-7 days.e. Depending on the medium used,growth will be indicated by increased turbidity, a color change,or both.f. Inoculate a Trypticase Soy Agar with 5% Defibrinated Sheep Blood Agar plate with 0.1 mL to check forcontamination.g. This strain starts to show growth in the first few dilution tubes within 72 hours. Additionalincubation may be required for growth on solid medium.h. Subsequent,fresh transfers grow in 24-48 hours. The freeze-drying process and thecryoprotectant occasionally slows the growth rate of the initial culture.
Storage conditions	2-8°C
Sharing mode	Public welfare sharing