BeNa Culture Collection

Hybridoma cells (anti-CD2)-BNCC
  • Hybridoma cells (anti-CD2)-BNCC
  • Hybridoma cells (anti-CD2)-BNCC
【OKT11】

Hybridoma cells (anti-CD2)

  • Price: $273
  • number:BNCC359687
  • Form:
    lymphoblast
Basic package DNA extraction
Package:
  • Package A:mycoplasma detection assay + 2 vials of frozen cell
  • Package B:mycoplasma detection assay + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Hybridoma cells (anti-CD2)
Culture medium IMDM complete medium: 80% IMDM + 20% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ and put it into PE gloves, quickly subtract into a water bath pot at 37 ℃, shake the frozen vial to accelerate dissolution, and completely dissolve within 1 minute. (2) Add the dissolved cell solution to 9ml in the ultra-clean table; In the centrifuge tube of complete culture medium, centrifuge at 1000-1200rpm/min for 5 minutes, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to a T25 flask containing 6-8mL complete medium and placed in an incubator for upright cultivation. Cell subculturing: ① Centrifugation: collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8ml of culture medium according to a ratio of 1:2. (2) Half-volume liquid medium exchange: after the culture flask is allowed to stand for 5-10 minutes, half of the supernatant medium is gently sucked away, the remaining medium with cell precipitation is resuspended and mixed evenly, and the cell suspension is divided into a fresh T25 flask containing 8ml of medium according to a ratio of 1:2. (3) Pay attention to the change of pH of medium and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 106 cells/ml.
Growth conditions Culture temperature 37 ℃; Atmosphere 5% CO2 + 95% air;
Growth characteristics Suspension growth
Storage conditions Liquid nitrogen
Safety level 1
morphology lymphoblast
Separation substrate Mouse B lymphocyte, mouse lymphoma cell
Sharing mode Public welfare sharing

OKT 11 hybridoma cells (anti-CD2)

BNCC number: 359687

Cell name: OKT 11 hybridoma cells (anti-CD2)

Growth properties : Suspension growth

Growth conditions: 37 ℃,5% CO2 complete medium: 80% IMDM + 20% FBS

Frozen storage conditions: 50% IMDM + 40% FBS + 10% DMSO

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 2-3h, and then carry out handling procedure. Under sterile conditions, transfer the culture solution in T25 flask to a centrifuge tube, centrifuge at 1000-1200rpm/min for 5 ~ 10 minutes, resuspend the cells, transfer the suspended cells to a fresh T25 flask, add 10ml of complete medium, and put them into the incubator.Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of loss of cell viability.

Recovery steps: 
(1)The frozen vial is taken out of liquid nitrogen or -80 ℃ refrigerator and put into PE gloves, quickly submersed into a 37 ℃ water bath, shaken the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; 
(2)Put the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 5min, discard the supernatant, resuspend the cells with 1-2ml of complete media. 
(3)The cell suspension was added to T25 flask containing 5-6ml complete medium and cultured in an incubator. 
Cell subculturing: 
(1) Centrifugation: collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8ml of culture medium according to a ratio of 1:2. 
(2) Half-volume solution exchange:  the culture flask is sit for 5-10 minutes, half of the supernatant medium is gently sucked away, the remaining medium with cell precipitation is resuspended and mixed evenly, and the cell suspension is dispensed into a fresh T25 flask containing 8ml of medium according to a ratio of 1:2. 
(3) Pay attention to the change of pH value of media and cell density, renew the media regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 10 ⁶ cells/ml.
Notes:
(1)The cells are density dependent. Initiate the subcultivation in T25 flask when the cell density reaches 80%; A subcultivation ratio of 1:2 is recommended. 
(2)If the cells in sealed culture bottle, put it into the incubator for cultivation after handling, with the cap unscrewed.
Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

Item quality standard Recovery record
viability: suspension growth rate ≥ 80.0% in 108 hours suspension growth rate reaches  30.0% in 18 hours, and 80.0% in 98 hours
cell morphology: suspension, lymphoblast suspension, lymphoblast, round
attached figure:
Conclusion: good viability, and no abnormal cell morphology, qualified
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